ABSTRACT
Background: The objective of this retrospective study was to compare the efficacy of slow freezing and Vitrification for the cryopreservation of supernumerary cleavage stage embryos on day 3 after IVF and its impact on clinical outcome. Methods: 485 supernumerary embryos of IVF cycles (from Oct 2011 to Dec 2012) were cryopreserved by slow freezing method while 502 embryos (from Jan 2013 to April 2014) by Vitrification method. 362/485 and 230/502 embryos were thawed for FET cycles (65 patients in each group).After warming the survival rate, post warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups. Results: There were 65 frozen thawed cycles in each group. The percentage of excellent and good morphology embryos before cryopreservation were same in both the groups, but after thawing the results were significantly in favour of Vitrification as compared to Slow freezing. In Vitrification group versus Slow freezing group, the different outcomes were survival rate (96.95% vs. 69.06%, p-0.000), post warmed excellent morphology embryos (94.17% vs. 60.8%, p-0.000) clinical pregnancy rate (41.53% vs. 21.53%, p-0.043) and the implantation rate (14.41% vs. 7.01%, p-0.024). Conclusions: Vitrification is a promising alternate to the conventional slow freezing method in terms of not only excellent survival and post warmed excellent morphology embryo rate but also higher clinical pregnancy and implantation rate.
ABSTRACT
Objective: To evaluate the effect of endometrial thickness, pattern and E2 level on the day of human chorionic gonadotropin (hCG) administration on clinical outcome in patients undergoing in vitro fertilization and embryo transfer (IVF-ET). Method: A total number of 112 cycles of IVF-ET conducted at Jaipur Fertility Centre, an Infertility Unit of Mahatma Gandhi University of Medical Sciences and Technology, Jaipur were observed prospectively. Endometrial ultrasonographic characteristics and E2 levels were recorded on the day of hCG administration. Clinical pregnancy rates were analyzed with different endometrial thickness and E2 levels. Results: Overall 37.5% patients conceived and in these women endometrial thickness was between 6-14 mm. Only 2.38% patients conceived with an ET of <6 mm. Maximum patients (52.33%) who conceived were with an ET of 8-10 mm. It was observed that endometrial thickness pattern did not have significant effect on clinical pregnancy rate in these cases. Clinical pregnancy rate was significantly higher in patients with serum E2 levels between 1,000-2,500 pg/mL as compared to other two groups. Conclusion: When thin endometrium ≤6 mm with no triple-line coexist and serum E2 level is >2,500 pg/mL in IVF/ICSI-ET, candidate cryopreservation should be recommended.
ABSTRACT
Prior to the development of intracytoplasmic sperm injection (ICSI), azoospermic and severely oligospermic men had little to no chance of having a biological child. In this study, ICSI outcome in 212 transfers with ejaculated spermatozoa and 44 transfers with surgically retrieved spermatozoa were evaluated. Material and methods: The 68 singleton gestations achieved by ICSI were segregated according to underlying infertility etiology, with 54.41% having male factor and 45.59% having female factor. None of the patients had coexisting infertility factor. Results: The miscarriage rate of all ICSI singleton gestations during the first trimester was 19.12%. There were no significant differences in early pregnancy loss (EPL) rate by infertility factor. Among patients undergoing ICSI because of male factor, there were no significant differences in EPL using ejaculated or nonejaculated sperm. Regardless of etiology, women aged ≥35 years had significantly higher EPL (36.36%). Conclusion: Our preliminary results demonstrate that first trimester miscarriage rates of ICSI gestations are not affected by underlying infertility etiology but are affected by maternal age.